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The temperature-sensitive period is from late L4 to early adult, a period just preceding the first signs of oogenesis. The finding of gain-of-function alleles which confer a phenotype opposite to that of loss-of-function alleles supports the idea that fem-3 plays a critical role in germ-line sex determination. Furthermore, the germ-line specificity of the fem-3 gf mutant phenotype and the late temperature-sensitive period suggest that, in the wild-type XX hermaphrodite, fem-3 is negatively regulated so that the hermaphrodite stops making sperm and starts making oocytes. Temperature shift experiments also show that, in the germ line, sexual commitment appears to be a continuing process.
Spermatogenesis can resume even after oogenesis has begun, and oogenesis can be initiated much later than normal. Selected References These references are in PubMed. This may not be the complete list of references from this article.
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The genetics of Caenorhabditis elegans. Mutations causing transformation of sexual phenotype in the nematode Caenorhabditis elegans. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Conditional-lethal Girlfriend fem for fem that suppress genetic defects in morphogenesis by altering structural proteins. Temperature-sensitive developmental mutants of Caenorhabditis elegans. Suppression of an amber mutation by microinjection of suppressor tRNA in C. Localized Negative Interference in Bacteriophage. Escherichia coli mutants with temperature-sensitive synthesis of DNA.
A new class of Escherichia coli recBC mutants: Cotransduction with thy of a gene required for genetic recombination in Escherichia coli. Mutant single-strand binding protein of Escherichia coli: Genetics and function of DNA ligase in Escherichia coli. Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA.